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Compact recirculating chiller with powerful 400 W cooling capacity, designed for simple cooling tasks down to -10 °C.
The RC 2 lite is the perfect peripheral device for the cooling of rotary evaporators, reflux condensers or as a cooling source for devices that dissipate heat with the help of a heat exchanger (e. g. double-walled grinding containers, cooled incubators, laboratory reactors, cooling coils).
The easily accessible and easy-to-clean mesh filter (stainless steel) ensure a consistently high cooling capacity over years. Thanks to the low filling volume of just one liter, the RC 2 lite is able to reach low temperatures particularly fast. The large working volume of 2.5 liters enables a high number of external applications without refill of liquids. The filling level in the reservoir can be clearly observed through the large, illuminated sight glass.
The powerful pressure and suction pump enables the simultanous operation of several small applications as well as external applications in open bath vessels in combination with a level controller. Thanks to the natural refrigerant R290, the RC 2 lite is remarkably environmentally friendly and well prepared for the future.
| Appliance type | Recirculating chiller |
| Class designation acc. DIN 12876 | I |
| Identification according to DIN 12876 | NFL |
| Cooling agent | R290 |
| Cooling agent quantity | 76 g |
| Cooling agent pressure max. | 21 bar |
| Cooling capacity (@20°C) | 400 W |
| Cooling capacity (@10°C) | 350 W |
| Cooling capacity (@0°C) | 250 W |
| Cooling capacity (@-10°C) | 140 W |
| Working temperature | -10 – room temp. °C |
| Operating temperature min. | -10 °C |
| Operating temperature max. (with external heating) | 70 °C |
| Temperature display | yes |
| Temperature control | PID |
| Working temperature sensor | PT1000 |
| Working temperature display | LED |
| Temperature stability DIN 12876 | ±0.5 K |
| Display resolution | 0.1 K |
| Set temperature resolution | 0.1 K |
| Filling volume | 1 – 3.5 l |
| Pump type | Pressure- / suction pump |
| Pump pressure max. (0 liters discharge flow) | 0.35 bar |
| Pressure pump (suction side) (0 liter flow) | 0.15 bar |
| Flow rate max. (0 bar back pressure) | 18 l/min |
| Pump connection | M16x1 |
| Permissible ON time | 100 % |
| Noise level | 51 dB(A) |
| Dimensions (W x H x D) | 225 x 385 x 430 mm |
| Weight | 24.5 kg |
| Permissible ambient temperature | 5 – 32 °C |
| Permissible relative humidity | 80 % |
| Protection class according to DIN EN 60529 | IP 21 |
| RS 232 interface | yes |
| USB interface | USB-B |
| Voltage | 230 V |
| Frequency | 50/60 Hz |
| Power input | 250 W |
Control and monitoring
The intuitive software allows easy control and monitoring of the bioprocess and associated parameters via a 10.4-inch tablet. Users can select from batch, fed-batch, and perfusion/continuous operating modes, catering to various cell culture techniques. The innovative Chaotic Mixing function enables efficient and homogeneous mixing when required. The control software provides clear calibration management, real-time data visualization, and complete process documentation, ensuring compliance with FDA CFR Part 11 requirements (optional).
Gas supply
Precise and adjustable gassing is achieved through four individual built-in mass flow controllers for four each separate gas line (N2, O2, air and CO2), enabling flow rates of 0.01 – 2 L / min. tailored to cell growth requirements. By using the integrated and freely customizable cascade control system, each gas flow can be automatically adjusted by the software at any time according to the ambient conditions in order to maintain the target parameters.
Liquid supply
Four integrated Watson-Marlow pumps with adjustable direction and speed offer highest precision and versatility in pumping in and out acid, base, anti-foam agents and feeding solutions for mammalian cell culture media management.
Sensors
HABITAT Bioreactor comes equipped with latest technology sensors to precisely monitor and control critical bioprocess parameters. The following sensors are included in our standard sales vessel packages:
• pH
• DO (dissolved oxygen)
• temperature
• filling level
• foam
Optional sensors: CO2, Conductivity, Turbidity, Viable cell mass, Redox, Off-gas analysis and more.
PID temperature control
The integrated PID control ensures precise temperature regulation at all times, regardless of vessel type (single-wall or double-wall) or fill level. Single-wall glass vessels can be heated with a heating blanket, allowing for accurate temperature settings above room temperature. With the optional use of a cooling finger, it is also possible to achieve temperatures below room temperature. For double-wall vessels, in combination with an IKA thermostat, the temperature range can be freely adjusted between 5-80°C, independent of room conditions or heat generated by the culture itself.
All features at a glance
• compact, space-saving design, dimensions: 223 x 402 x 450 mm (WxDxH)
• latest technology sensors for better insights and more control
• large screen tablet for easy operation (10.4 inch)
• intuitive, easy-to-use control software that provides simple initial setup and intuitive user assistance throughout the process
• four integrated Watson-Marlow pumps
• high precision motor speed control
• cross platform connectivity: USB, PC, RS232, OPC-UA, ethernet, external signal input, external pump, single-use unit, thermostat
• data storage
• four built-in mass flow controllers for gas supply: N2, O2, air, and CO2
• status LED display: direct error display by indicator light
Note: Only a control tower and the separately available vessel package make up a functional unit.
Scope of delivery
| Controller | Cell Growth |
| Operating mode | timer, continuous and program operation |
| Display | Tablet PC |
| Operation | touch screen |
| Data memory size | 32 GB |
| Auto restart after power failure | yes |
| Ethernet interface | yes |
| Single use reactor interface | yes |
| Thermostat interface | yes |
| Ext. pump interface | yes |
| Gravimetric feeding | yes |
| Exponential feeding | yes |
| Biomass feeding | yes, with Excell Sensor |
| OPC UA interface | yes |
| Filter heater | yes |
| LED light panel | Accessory |
| Stirring motor | yes |
| Calibration guide temperature | yes |
| Calibration guide pH | yes |
| Calibration guide pO2 | yes |
| pH – temperature compensation | yes |
| Mode hose filling | yes |
| Stirrer control mode | off, manual, profile, Cascade-pO2 control, chaotic |
| Speed range | 1 – 2200 rpm |
| Speed deviation >= 100 rpm | ±2 % |
| Speed deviation <= 100 rpm | ±5 rpm |
| Speed display | TFT |
| Reversible direction of rotation | yes |
| Timer | yes |
| Timer display | TFT |
| Time setting range | 1 – 120 min |
| Heating blanket | yes |
| Heat output | 250 W |
| Heat control | TFT |
| Temperature control | PID |
| Working temperature | 5 – 80 °C |
| Connection for ext. temperature sensor | PT1000 |
| Temperature display | yes |
| Temperature measuring range | 0 – 100 °C |
| Temperature measurement resolution | 0.1 K |
| Temperature control accuracy | ±0.2 °C |
| Temperature limit min. | 0 °C |
| Temperature limit max. | 110 °C |
| pH measurement | yes |
| pH control mode | off, auto, profile |
| pH value display | TFT |
| pH measuring range | 0 – 14 pH |
| pH measurement resolution | 0.01 pH |
| Accuracy of pH measurement | ±0.02 pH |
| Set resolution pH value | 0.01 pH |
| pH control accuracy | ±0.05 pH |
| Conncetion for pO2 sensor | yes |
| pO2 control mode | off, manual, auto, profile |
| Measurement range pO2 min. | 0 %sat |
| Measurement range pO2 max. | 200 %sat |
| Resolution pO2 measurement | 0.01 %sat |
| Accuracy of pO2 measurement | ±0.3 %sat |
| pO2 control accuracy | 0.3 %sat |
| Exhaust Gas Analyzer | yes, with Blue Vary Sensor |
| OUR,CER,RQ calculation | yes, with Blue Vary Sensor |
| RQ feeding | yes, with Blue Vary Sensor |
| Digital pH sensor | yes, with EasyFerm Bio Arc Sensor |
| Measuring Range min. digital pH | 0 pH |
| Measuring Range max. digital pH | 14 pH |
| Accuracy digital pH | ±0.02 pH |
| Digital DO sensor | yes, with OxyFerm FDA Arc |
| Measuring Range min. digital DO | 0 %sat |
| Measuring Range max. digital DO | 200 %sat |
| Accuracy digital DO | ±2 % |
| Optical DO sensor | yes, with VisiFerm RS485-ECS |
| Measuring Range min. optical DO | 0 %sat |
| Measuring Range max. optical DO | 300 %sat |
| Accuracy optical DO | ±1 % |
| Biomass sensor | yes, with CGQ BioR Sensor |
| Measuring Range min. Biomass | 0.5 OD |
| Measuring Range max. Biomass | 50 OD |
| Digital Redox / ORP sensor | yes, with EasyFerm Plus ORP Arc |
| Measuring Range min. digital ORP | -1500 mV |
| Measuring Range max. digital ORP | 1500 mV |
| Digital biomass sensor | yes, with Excell Sensor |
| Measuring Range min. digital biomass | 0 AU |
| Measuring Range max. digital biomass | 6 AU |
| Accuracy digital biomass | ±1 % |
| Viable Cell Density Sensors | yes, with Incyte Sensor |
| Measuring Range min. cell density | 5 x 10^5 cells/mL |
| Measuring Range max. cell density | 8 x 10^9 cells/mL |
| Accuracy cell density | ±1 % |
| Gas in connection Air | 6 mm |
| Gas out connection Air | 6 mm |
| Gas in connection O2 | 6 mm |
| Gas out connection O2 | 6 mm |
| Gas in connection N2 | 6 mm |
| Gas out connection N2 | 6 mm |
| Gas in connection CO2 | 6 mm |
| Gas out connection CO2 | 6 mm |
| Aeration mode | continuous & automatic |
| Display flowrate | l/min, cc/min |
| Input pressure | 200 – 3000 mbar |
| Pressure out min. | 200 mbar |
| Pressure out max. | 1000 mbar |
| Flowrate Air min. | 10 cc/min |
| Flowrate Air max. | 2000 cc/min |
| Flowrate O2 min. | 10 cc/min |
| Flowrate O2 max. | 2000 cc/min |
| Flowrate N2 min. | 10 cc/min |
| Flowrate N2 max. | 2000 cc/min |
| Flowrate CO2 min. | 10 cc/min |
| Flowrate CO2 max. | 2000 cc/min |
| Measuring and control accuracy of the gas control units | ±2 % fsr |
| Peristaltic pump | yes |
| Numbers of pump heads | 4 |
| Pump head function | Acid, Base, AntiFoam, Level |
| Pump head | Watson Marlow 114DV |
| Number of pump tubes | 4 |
| Number of pump rollers | 4 |
| Flow rate min. | 0.3 ml/min |
| Flow rate max. | 270 ml/min |
| Flow rate control accuracy | ±0.3 ml/min |
| Pump Speed min. | 33 rpm |
| Pump Speed max. | 400 rpm |
| Pump reversible direction of rotation | yes |
| Pump speed display | TFT |
| Inner tube diameter min. | 0.5 mm |
| Inner tube diameter max. | 4.8 mm |
| Tube outer diamter min. | 4.1 mm |
| Tube outer diamter max. | 8.4 mm |
| Tube material | Silicone |
| Tube hardness | 50 – 60 Shore A |
| Dimensions (W x H x D) | 224 x 624 x 405 mm |
| Weight | 14 kg |
| Packing unit | 1 pcs |
| Permissible ambient temperature | 5 – 40 °C |
| Permissible relative humidity | 80% (up to 31°C), decreasing linearly to max. 50 (@40°C) |
| Protection class according to DIN EN 60529 | IP 20 |
| RS 232 interface | yes |
| USB interface | USB-B |
| WiFi Interface | yes |
| Voltage | 100 – 240 V |
| Frequency | 50/60 Hz |
| Power input | 300 W |
| Power input max. | 1100 W |
| Power input standby | 10 W |
| Fuse | 2x T5.0A 250V |

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